TOKYO UNIVERSITY OF SCIENCE TAKAHASHI TSUJI'S LABORATORY

Preparation of dissociated single cells from epithelial and mesenchymal tissues of incisor tooth germ

At first, we isolated tooth germ at cap stage from the lower jaw in ED14.5 mice by microsurgery. To precisely replicate tooth organogenesis in early developmental stages, a cell aggregation method using cap stage tooth germ-derived epithelial cells and mesenchymal cells was chosen. The purity of the isolated germ is essential to keep the high frequency of the generation of a bioengineered tooth. The isolated tooth germs were treated with enzyme solution. Then, the tissues were separated using a fine needle. The single cells of each tissues were prepared by the further enzymatic treatments.