TOKYO UNIVERSITY OF SCIENCE TAKAHASHI TSUJI'S LABORATORY

A GFP-labeled bioengineered tooth in the oral environment of adult mice

We also transplanted green fluorescence protein (GFP)-labeled bioengineered tooth germ, which was reconstituted by normal epithelial cells and the mesenchymal cells from GFP-transgenic mice into non-transgenic mice. A GFP-labeled bioengineered tooth was produced and could be observed in the bony hole in the alveolar bone of adult mice. GFP-positive mesenchymal cells were detectable both in the odontoblasts and in the dental pulp and PDL, which differentiate from the dental papilla and dental follicle cells, respectively. Green fluorescence was also observed in the dentinal tubules of the GFP-positive odontoblasts in the regenerated tooth.