TOKYO UNIVERSITY OF SCIENCE TAKAHASHI TSUJI'S LABORATORY

Histology of the bioengineered tooth

We next evaluated the tooth tissue structure of the bioengineered tooth during eruption process.
The bioengineered tooth also formed a correct structure comprising enamel, ameloblast, dentin, odontoblast, dental pulp, alveolar bone, and blood vessels. It is known that mice have a considerable amount of cellular cementum which increases in thickness both on the sides of the roots and in the interradicular area and forms around the apex of the molar roots. The fully occluded bioengineered tooth was also observed to have a large amount of cellular cementum that was equivalent to a normal murine molar tooth. The root of the bioengineered tooth was also observed to be surrounded by sufficient PDL.
Observations of the bioengineered tooth morphology revealed that the crown had plural cusp structure. These results indicated that the tooth tissue structures of the bioengineered tooth was similar to those of a normal tooth.